A standard curve is
a graph relating a measured quantity (e.g., radioactivity,
fluorescence, or optical density) to concentration of the
substance of interest in "known" samples. You prepare and
assay "known" samples containing the substance in amounts
chosen to span the range of concentrations that you expect
to find in the "unknown" samples. You then draw the standard
curve by plotting assayed quantity (on the Y axis) vs. concentration
(on the X axis). Such a curve can be used to determine concentrations
of the substance in "unknown" samples.
Use the graph below to answer this question:
What is the concentration of DNA in a sample with Abs280
of 1.5? Answer |