Hopkin's rose
Nudebranch, California coast.
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Biology 230

Isolation of DNA

(BIOL 230, Lab Experiment 17)

  BIOL 230 lab contents   BIOL 230   My home page
 
   

DNA can be precipitated in cold ethanol and collected on a glass rod.

How did you lyse the cells to extract their DNA: physical or chemical methods?

 

A standard curve is a graph relating a measured quantity (e.g., radioactivity, fluorescence, or optical density) to concentration of the substance of interest in "known" samples. You prepare and assay "known" samples containing the substance in amounts chosen to span the range of concentrations that you expect to find in the "unknown" samples. You then draw the standard curve by plotting assayed quantity (on the Y axis) vs. concentration (on the X axis). Such a curve can be used to determine concentrations of the substance in "unknown" samples.

Use the graph below to answer this question:
What is the concentration of DNA in a sample with Abs280 of 1.5? Answer

 

Class DNA Standard Curves are on WebAccess.

 

The answer is 144 µg/mL.