Fermenters used for the production of glutamic acid for MSG. Each fermenter holds 63,420 gallons and is ~100 ft tall. Hofu, Japan. For what is MSG used?

Biology 230

Industrial Fermentation

(BIOL 230, Lab Experiment 15)

BIOL 230 lab contents BIOL 230 My home page
 

Antibiotic test & Endospore stain
Review questions
Benchtop fermenter
Some sample class data (graphs)
Bacitracin structure

Class data in Canvas

After the semester is over, read a mystery-thriller from the New York Times bestseller list -- a fermenter is essential in Robin Cook's Vector (1999)

   
 


Computers are used to monitor conditions in this 15-L bioreactor.


S. aureus is inhibited by the bacitracin that diffused from the disk.
 

In this stain, green endospores are in red cells; there is one endospore per cell.


In this stain, most of the green endospores have been released from cells. The remaining cells are red. The endospore at the arrow is inside a cell.
In this stain, red endospores are in coloreless cells.

In your stains, endospores appear as green objects inside red cells. In older cultures, the sporangia (cells) will disintegrate leaving the endospores.
What is the function of endospores?
How do they differ from fern spores (Lab. #2)?

Back to top