Question 1: Use the data from three bioreactors shown below to answer the questions:
	A. What went wrong with Bioreactor #1?
	B. Which (bioreactor #2 or #3) is an example of a secondary metabolite?

Question 2. You have isolated a bacterium that produces a bacteriocin against Salmonella. The bacteria grow well in Medium 1 but don't produce bacteriocin. Testing one component at a time, you determine that iron interferes with bacteriocin production. When you try Medium 1 without iron, the bacteria don't grow. Bacteriocin production is best in Medium 2. Design a procedure to maximize the biomass and the yield of the bacteriocin.

Medium 1 Glucose 0.50% Citric acid 0.10% 20 amino acids 0.04% K2HPO4 0.15% MgSO4 0.12% FeSO4 1.54 x 10--5%

Medium 2 Glycerol 3.50% Glucose 0.50% Cirtic acid 0.10% 20 amino acids, each 0.04% K2HPO4 0.15% MgSO4 0.12%

Question 3. What are bacteriocins?

Question 4. How might a bacteriocin against Salmonella be used?