Lysozyme is an enzyme produced by many cells. In humans, it is found in perspiration, tears, mucus, and saliva. Lysozyme is particularly active on the major cell wall components of most gram-positive bacteria, making them vulnerable to lysis. Lysozyme catalyzes hydrolysis of the bonds between the sugars in the repeating disaccharide “backbone” of peptidoglycan. This act is analogous to cutting the steel supports of a bridge with a cutting torch: the gram-positive cell wall is almost completely destroyed by lysozyme. The cellular contents that remain surrounded by the plasma membrane may remain intact if lysis does not occur; this wall-less cell is termed a protoplast . Typically, a protoplast is spherical and is still capable of carrying on metabolism. Lysozyme assay
-Do these plants make lysozyme?
-What part(s) of plant? pH range compared to standard egg white lysozyme?
-Action against gram+, gram-, yeast?
-What is your control?