Fruit bat.

Biology 230

Research Project


Lysozyme is an enzyme produced by many cells. In humans, it is found in perspiration, tears, mucus, and saliva. Lysozyme is particularly active on the major cell wall components of most gram-positive bacteria, making them vulnerable to lysis. Lysozyme catalyzes hydrolysis of the bonds between the sugars in the repeating disaccharide “backbone” of peptidoglycan. This act is analogous to cutting the steel supports of a bridge with a cutting torch: the gram-positive cell wall is almost completely destroyed by lysozyme. The cellular contents that remain surrounded by the plasma membrane may remain intact if lysis does not occur; this wall-less cell is termed a protoplast . Typically, a protoplast is spherical and is still capable of carrying on metabolism. Lysozyme assay

-Do these plants make lysozyme?
-What part(s) of plant? pH range compared to standard egg white lysozyme?
-Optimum temp?
-Temp range?
-Action against gram+, gram-, yeast?
-What is your control?

Possible plants:

Baccharis pilularis
Delairea odorata
Eriophyllum staechadifolium
Cornus sericea
Sequoia sempervirens

Cultures available:
Aspergillus sp.
Candida spp.
Escherichia coli
Micrococcus lysodeikticus
Salmonella enterica
Shigella sonnei
Staphylococcus aureus
Saccharomyces cerevisiae
Pseudomonas aeruginosa
Streptococcus pneumoniae
Streptococcus pyogenes
Streptococcus mutans