1. CELL
BEHAVIOR AND SLIME MOLDS
Use of low-power microscopy and metric measures to study the
behavior of a cell
2. FERN DEVELOPMENT
Study of photomorphogenesis
3. CELL
STRUCTURE AND MICROSCOPY
Use of the compound light microscope, microscopic measurements,
and differential staining to study cell structure in wet mounts
4. TISSUE
PRINTING
Tissue printing is used to detect peroxidase in plants
5. MUSCLE
CELLS
Use of microscopic measurements to study muscle cell contraction
6. BIOLOGICALLY IMPORTANT ORGANIC
MOLECULES
An introduction to chemical analyses including indicator systems
and paper chromatography used to study cellular metabolism
7. GEL FILTRATION
Use of column chromatography to separate products
8. DIFFUSION AND OSMOSIS
Weighing, pipetting, and percent-difference calculations are
used to study properties of the plasma membrane
9. MEMBRANE RECEPTORS
Lectins are used to identify sugars on human cells
10. LACTATE
DEHYDROGENASE ISOENZYMES
Differentiation of proteins by gel electrophoresis
11. ENZYME
KINETICS
An investigation of catalase activity using computerized data
collection; Km determination for acetylcholinesterase using
a computer simulation and graphing
12. PHOTOSYNTHESIS
Cell fractionation, super-speed centrifugation, and spectrophotometry
are employed to study light reactions and light-trapping pigments
13. CELLULAR
METABOLISM
Aerobic and anaerobic bacterial culture techniques are used
to investigate hydrolysis, fermentation, enzyme induction, and
respiration
14. MITOCHONDRIA
Mitochondria for spectrophotometric assays of succinate dehydrogenase
and electron transport chain reactions are obtained by cell
fractionation
15. INDUSTRIAL
FERMENTATION
A bioreactor is used to produce an antibiotic
16. GENETIC ENGINEERING
OF PLANTS
Aseptic techniques and cell culture are used to investigate
protoplast fusion, transformation by Agrobacterium, and
cloning
17. ISOLATION OF DNA
Cell fractionation and chemical precipitation are used to isolate
DNA. Concentration of DNA is determined by spectrophometry
18. DNA FINGERPRINTING
RFLPs are separated by gel electrophoresis
19. RECOMBINANT DNA
& TRANSFORMATION
Restriction enzyme digests and plasmid ligation precede transformation
of E. coli
20. POLYMERASE CHAIN
REACTION
PCR is used to analyze human chromosomes
21. THE AMES TEST FOR
CHEMICAL CARCINOGENS
Bacterial cultures are used to assay for mutagenic chemicals
22. BIOREMEDIATION
Hydrocarbon-degrading bacteria are enumerated in environmental
samples
Appendix B. MICROSCOPIC MEASUREMENTS
Appendix E. ASEPTIC TECHNIQUES
Centrifuge conversions |
BIOL
230 lab contents