Seastar. New Zealand.
Photo©CLCase

Biology 215

Microscopy

Christine Case
 

formulaTo estimate diameter of the field:
DH = diameter for high power objective
DL = measured diameter under low power
M = magnification

 

Key to algae
Algae in Water Supplies
Protozoa kay



Scanning power (40X)

Use scanning power to locate and center the objects. You may not be able to distinguish much detail at this point. It's okay to use an air bubble (black circle on the left) for focusing. After you have focused the slide with the scanning objective, switch to the low power lens and focus using the fine adjustment. Then move on to the oil-immersion lens to see the bacteria.


Low power (100X)

Use low power to center and focus the image. After you have focused the image with low power, switch to the high-dry lens and focus using the fine adjustment.


High-dry objective (450X)

Focus using the fine adjustment. The cells are more defined but intracellular detail cannot be seen. Move on to the oil-immersion lens to see more detail.


Oil-immersion objective (1000X)

Place a drop of oil on the slide; the lens sits in the oil. Focus using the fine adjustment. Now you can see the euglenoid's chloroplasts and red pigment that migrate to the surface when it's warm.

Photos©CLCase