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Biology 230

Polymerase Chain Reaction

(BIOL 230, Lab Experiment 20)

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Using PCR

A 22-year-old man presented to an emergency department with a one-day history of fever, myalgia, chills, headache, and malaise; he did not have dyspnea or cough. The patient had been in excellent health. His temperature was 39.4°C, his pulse rate was 118 beats per minute, his blood pressure was 127/84 mm Hg, and his respiratory rate was 24 breaths per minute. His blood O2 level was 91%, determined by pulse oximetry.

The patient was discharged with no diagnosis or therapy. The day after dishcarge, the patient had acute respiratory failure and cardiopulmonary arrest and could not be resiscitated.

PCR was performed on tissue samples taken post-mortem. Which of the following primers do you want to use for PCR?
Yersinia pestis (plague)
Legionella pneumophila (legionellosis)
Hantavirus (Hantavirus pulmonary syndrome)
Francisella tularensis (tularemia)

After looking at the PCR results, go to the next question:Next