Christine Case, Skyline College

Serratia marcescens
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Visual Lab Study Guide

These pages are designed to help you interpret your lab results and to understand microbial physiology. The photographs on these pages cannot replace the experience gained from doing the experiments.

1. Use and Care of the Microscope	Observing bacteria
2. Examination of Lliving Microorganisms	Wet mount
3. Microbes in the Environment	Bacterial and fungal colonies Nutrient broth
4. Transfer of Bacteria: Aseptic Technique	Culture media Motility in a deep Lactococcus
5. Preparation of Smears	Notes
6. Negative stain	Stain
7. Gram Stain	Interpreting a Gram stain
8. Acid Fast Staining	Interpreting an acid-fast stain
9. Structural Stains	Capsule stain Endospore stain
10. Morphologic Unknown	Constructing a dichotomous key
11. Isolation of Bacteria by Dilution Techniques	Pour plates Find the pure culture Streptomyces
12. Special Media for Isolating Bacteria	MSA EMB
13. Carbohydrate Catabolism	Starch hydrolysis OF medium
14. Fermentation of Carbohydrates	MR test VP test Fermentation tubes Citrate
15. Protein Catabolism, Part 1	Urea hydrolysis Gelatin hydrolysis Litmus milk
16.Protein Catabolism, Part 2	Indole test Peptone iron agar MIO Phenylalanine deaminase
17.Respiration	Oxidase test Nitrate reduction Catalase test
18. Unknown Identification and Bergey's Manual	Unknown practice
19. Oxygen and the Growth of Bacteria	Thioglycollate
20. Determination of a Bacterial Growth Curve	Sample graphs Critical Thinking Qu. #2 Cell numbers and Abs.
21. Biofilms	In nutrient broth
22. Physical Methods of Control: Heat Class data	Graph of sample results The Demonstration Plates
23. Physical Methods of Control: Ultraviolet Radiation	UV radiation
24. Chemical Methods of Control: Disinfectant and Antiseptics	Sample results
25. Chemical Methods of Control: Antimicrobial Drugs	Antibiosis Kirby-Bauer Test
26. Effectiveness of Hand Scrubbing	Sample results
27. Regulation of Gene Expression	Green Fluorescent Protein Nitrate reductase lac operon
28. Isolation of Bacterial Mutants	Serratia results
29. Transformation of Bacteria	Isolation of DNA Transformation
30. DNA Fingerprinting	RFLPs
31. Genetic Engineering	Recombinant DNA
32. Ames Test for Detecting Possible Chemical Carcinogens	Ames Test
33. Fungi: Yeasts and Moldes	Yeast \| Aspergillus
34. Phototrophs: Algae and Cyanobacteria	Algae
35. Protozoa	Photos
36. Parasitic Helminths	Helminth eggs
37. Isolation of Bacteriophages	Bacteriophage
38. Plant Viruses	Tomato
39. Epidemiology \| Data	Serratia Epidemiology
40. Koch's Postulates	Koch's Postulates Soft rot Milky spore disease
41. Innate Immunity	Lysozyme
42. Agglutination Reactions: Slide Agglutinations	Blood typing
43. Agglutination Reactions: Mictotiter Agglutinations	Sample results & Self-quiz
44. ELISA Technique	Sample results
45. Bacteria of the Skin	Coagulase test Mannitol salt agar Tellurite glycine agar
46. Bacteria of the Respiratory Tract	Blood agar
47. Bacteria of the Mouth	MSB Agar
48. Bacteria of the Gastrointestinal Tract	MacConkey agar TSI slant PEA
49. Bacteria of the Urogenital Tract	Pseudomonas Neisseria gonorrhoeae
50. Identification of an Unknown from a Clinical Sample	The assignment Unknown practice
51. Rapid Identification Methods	Citrate IMViC Enterotube API Differentiation of Enterobacteriaceae.pdf
52. Microbes in Water: Multiple Tube Technique	MPN Test \| Class data MUG agar
53. Microbes in Water: Membrane Filter Techniques	Endo and Enterococcus agar \| Class data
54. Microbes in Food: Contamination	Standard Plate Count
55. Microbes Used in the Production of Foods	Beer Chocolate
56. Microbes in Soil: The Nitrogen and Sulfur Cycles	Winogradsky column Nitrate reduction tests Root nodules
57. Microbes in Soil: Bioremediation	Lipid hydrolysis Enrichments
Serial Dilutions	About serial dilutions Appendix B answers