1. Use and Care of the Microscope |
Observing bacteria
|
2. Examination of Lliving Microorganisms |
Wet mount |
3. Microbes in the Environment |
Bacterial
and fungal colonies
Nutrient broth |
4. Transfer of Bacteria: Aseptic Technique |
Culture media
Motility in a deep
Lactococcus |
5. Preparation of Smears |
Notes |
6. Negative stain |
Stain |
7. Gram Stain |
Interpreting a Gram
stain |
8. Acid Fast Staining |
Interpreting an acid-fast
stain |
9. Structural Stains |
Capsule stain
Endospore stain |
10. Morphologic Unknown |
Constructing
a dichotomous key
|
11. Isolation of Bacteria by Dilution
Techniques |
Pour plates
Find the pure culture
Streptomyces |
12. Special Media for Isolating Bacteria |
MSA
EMB |
13. Carbohydrate Catabolism |
Starch hydrolysis
OF medium |
14. Fermentation of Carbohydrates |
MR test
VP test
Fermentation tubes
Citrate |
15. Protein Catabolism, Part 1 |
Urea hydrolysis
Gelatin hydrolysis
Litmus milk |
16.Protein Catabolism, Part 2 |
Indole test
Peptone iron agar
MIO
Phenylalanine deaminase |
17.Respiration |
Oxidase test
Nitrate reduction
Catalase test |
18. Unknown Identification and Bergey's Manual |
Unknown practice
|
19. Oxygen and the Growth of Bacteria |
Thioglycollate |
20. Determination of a Bacterial Growth
Curve |
Sample graphs
Critical Thinking Qu. #2
Cell numbers and Abs. |
21. Biofilms |
In nutrient broth |
22. Physical Methods of Control: Heat
Class data
|
Graph of sample results
The Demonstration Plates |
23. Physical Methods of Control: Ultraviolet
Radiation |
UV radiation |
24. Chemical Methods of Control: Disinfectant
and Antiseptics |
Sample results |
25. Chemical Methods of Control: Antimicrobial
Drugs |
Antibiosis
Kirby-Bauer Test |
26. Effectiveness of Hand Scrubbing |
Sample results |
27. Regulation of Gene Expression |
Green Fluorescent
Protein
Nitrate reductase
lac operon |
28. Isolation of Bacterial Mutants |
Serratia results |
29. Transformation of Bacteria |
Isolation of DNA
Transformation |
30. DNA Fingerprinting |
RFLPs |
31. Genetic Engineering |
Recombinant DNA |
32. Ames Test for Detecting Possible
Chemical Carcinogens |
Ames Test |
33. Fungi: Yeasts and Moldes |
Yeast | Aspergillus |
34. Phototrophs: Algae and Cyanobacteria |
Algae |
35. Protozoa |
Photos |
36. Parasitic Helminths |
Helminth eggs |
37. Isolation of Bacteriophages |
Bacteriophage |
38. Plant Viruses |
Tomato |
39. Epidemiology | Data |
Serratia
Epidemiology |
40. Koch's Postulates |
Koch's Postulates
Soft rot
Milky spore disease |
41. Innate Immunity |
Lysozyme |
42. Agglutination Reactions: Slide Agglutinations |
Blood typing
|
43. Agglutination Reactions: Mictotiter
Agglutinations |
Sample results
& Self-quiz |
44. ELISA Technique |
Sample results |
45. Bacteria of the Skin |
Coagulase
test
Mannitol salt agar
Tellurite glycine agar |
46. Bacteria of the Respiratory Tract |
Blood agar |
47. Bacteria of the Mouth |
MSB Agar |
48. Bacteria of the Gastrointestinal
Tract |
MacConkey agar
TSI slant
PEA |
49. Bacteria of the Urogenital Tract |
Pseudomonas
Neisseria gonorrhoeae |
50. Identification of an Unknown from
a Clinical Sample |
The assignment
Unknown practice
|
51. Rapid Identification Methods |
Citrate
IMViC
Enterotube
API
Differentiation of Enterobacteriaceae.pdf |
52. Microbes in Water: Multiple Tube
Technique
|
MPN Test | Class data
MUG agar |
53. Microbes in Water: Membrane Filter
Techniques |
Endo and Enterococcus
agar | Class data |
54. Microbes in Food: Contamination |
Standard
Plate Count |
55. Microbes Used in the Production of
Foods |
Beer
Chocolate |
56. Microbes in Soil: The Nitrogen and
Sulfur Cycles |
Winogradsky
column
Nitrate reduction tests
Root nodules |
57. Microbes in Soil: Bioremediation |
Lipid hydrolysis
Enrichments |
Serial Dilutions |
About serial
dilutions
Appendix B answers |