Great blue heron, San Francisco Bay
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Biology 230

Biologically Important Organic Molecules

(BIOL 230, Lab Experiment 6)

  BIOL 230 lab contents   BIOL 230   My home page
 

Glucose See glucose and its reducing power.
Calories Package labels and calculations.
Lipids

 

   

Amino acids

 

Calculate the Rf value for each spot then compare the unknown with the known amino acids to identify the unknowns.

What is the identity of the unknown in the sample shown at the right?

 

Rf

Amino acid 1

0.25

Amino acid 2

0.33

Unknown

0.25 & 0.33


Rf=sample migration/solvent migration

 Chromatogram

Lipids

 

 

The presence of red droplets after the addition of Sudan IV indicates the presence of lipids. Tube B is positive.

Sudan test 
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