Using the maps below, answer
the following questions about the gel:
1. Which restriction fragment
of pAMP contains the amp resistance gene?
2. Which restriction fragment of pKAN contains the kan
resistance gene?
3. Which restriction fragment is 784 bp?
4. Why are the pKAN fragments so close together?
What can you conclude from
these plates? The original culture was ampsE.
coli that is now growing on amp-ara LB agar.
The entire amp-ara-LB agar surface was inoculated with
E. coli. How do you explain the ampsE.
coli (white satellite colonies) growing only around
the red fluorescent E. coli??
BIOL
230 lab contents
